KMID : 1377020160130050579
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Tissue Engineering and Regenerative Medicine 2016 Volume.13 No. 5 p.579 ~ p.584
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Prefabrication of axially vascularized bone by combining ¥â-tricalciumphosphate, arteriovenous loop, and cell sheet technique
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Dongyang Ma
Liling Ren Zhen Cao Jianxue Li Jian Cao Wenyan Tian Hong Yao
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Abstract
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The repair of bone defects poses a great challenge for reconstructive surgeons. Although the development of ti©¬sue engineering has exhibited promise in replacing damaged bone, the fabrication of large constructs with functional blood ve©¬sels remains an obstacle. From the orthopedic surgeon¡¯s point of view, the generation of axially vascularized bone, which can anastomose with the recipient vessel, might be a solution to this medical problem. In this study, we aimed to prefabricate an axially vascularized bone by combining a ©¬-TCP scaffold, arteriovenous loop (AVL), and cell sheet in a bioreactor in vivo. Twelve rabbits were randomly allocated into two groups: the experimental group (presence of AVL) and the control group (absence of AVL). The constructs were explanted at 8 weeks postoperatively. The histomorphometric results showed 42.8¡¾5.9% of the bone area in the AVL group and 26.6¡¾3.5% in the control group. Similarly, vessel analysis revealed the average vessel density in the AVL group (12.5¡¾3.3) was significantly more than that in the control group (6.1¡¾1.5, p<0.05). Our research indicated that the combination of a ©¬-TCP scaffold, AVL and cell sheet might engineer vascularized bone. This prefabrication strategy might facilitate clinical translation of bone tissue engineering in reconstructing large bone defects.
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KEYWORD
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Prefabrication, Vascularization, Tissue engineered bone, Arteriovenous loop, Cell sheet
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